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PMTV-Diagnostic

Diagnostic methods for detecting potato mop-top virus approved by the Chief Plant Biosecurity Officer, WA 

Primers: PMTV-1948F, PMTV-2017R, and PMTV-1970 (Mumford et al., 2000) 

Mumford, R., et al. (2000). "Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay." Phytopathology 90(5): 448–453.

https://doi.org/10.1094/PHYTO.2000.90.5.448 

Primers: PMTV 401F, 404R, and 403P (Pandey et al., 2020)

Pandey, B., et al. (2020). "Development and application of a real-time reverse-transcription PCR and droplet digital PCR assays for the direct detection of Potato mop top virus in soil." Phytopathology 110(1): 58–67.

https://doi.org/10.1094/PHYTO-05-19-0185-FI

Primers: TGB-F, TGB-R, and TGB-P (Chikh-Ali et al., 2025)

Chikh-Ali, M., et al. (2025). "Development of a duplex immunocapture reverse-transcription quantitative PCR for large-scale detection of potato mop-top virus in dormant potato tubers." Plant Disease 109(6): 1354–1358.

https://doi.org/10.1094/pdis-11-24-2288-re